cell signaling technology cdk6  (Cell Signaling Technology Inc)

Journal: Molecular cell

Article Title: Mutant p53 Gains Its Function via c-Myc Activation upon CDK4 Phosphorylation at Serine 249 and Consequent PIN1 Binding

doi: 10.1016/j.molcel.2017.11.006

Figure Lengend Snippet: KEY RESOURCES TABLE

Article Snippet: ​ REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Mouse monoclonal anti-p53 (DO-1) Santa Cruz Biotechnology Cat# sc-126 AB_628082 Mouse monoclonal anti-p53 (DO-1) Santa Cruz Biotechnology Cat# sc-126 X Rabbit polyclonal anti-p53 (FL-393) Santa Cruz Biotechnology Cat# sc-6243 AB_653753 Normal mouse IgG Santa Cruz Biotechnology Cat# sc-2025 AB_737182 Rabbit monoclonal anti-GAPDH Cell Signaling Technology Cat# 5174 AB_10622025 Rabbit monoclonal anti-Histone H3 Cell Signaling Technology Cat# 4499 AB_10544537 Mouse monoclonal anti-cdc2 (CDK1) Cell Signaling Technology Cat# 9116P AB_2074795 Rabbit monoclonal anti-CDK2 Cell Signaling Technology Cat# 2546P AB_2276129 Rabbit monoclonal anti-CDK4 Cell Signaling Technology Cat# 12790P AB_2631166 Rabbit monoclonal anti-CDK6 Cell Signaling Technology Cat# 13331P Mouse monoclonal anti-CDK7 Cell Signaling Technology Cat# 2916P AB_10827986 Rabbit monoclonal anti-CDK9 Cell Signaling Technology Cat# 2316P AB_2291505 Mouse monoclonal anti-CDK4 Santa Cruz Biotechnology Cat# sc-56277 AB_1121419 Rabbit polyclonal anti-Phosphorylation of S249 This paper N/A Mouse monoclonal anti-Flag M2 Sigma-Aldrich Cat# F3165 AB_259529 Mouse monoclonal anti-Cyclin D1 Santa Cruz Biotechnology Cat# sc-20044 AB_627346 Mouse monoclonal anti-Cyclin D3 Cell Signaling Technology Cat# 2936P AB_10841292 Rabbit polyclonal anti-Pin1 Cell Signaling Technology Cat# 3722S AB_10692654 Rabbit polyclonal anti-Pin1 Santa Cruz Biotechnology Cat# sc-15340 AB_2237080 Mouse monoclonal anti-Pin1 Santa Cruz Biotechnology Cat# sc-46660 AB_628132 Rabbit monoclonal anti-PARP Cell Signaling Technology Cat# 9532S AB_10695538 Rabbit monoclonal anti-c-Myc Abcam Cat# ab32072 AB_731658 Mouse monoclonal anti-GFP Santa Cruz Biotechnology Cat# sc-9996 AB_627695 Mouse monoclonal anti-P21 WAF1 NeoMarkers Cat#MS-891-P1 Mouse monoclonal anti-beta-Actin Sigma-Aldrich Cat# A1978 AB_476692 Mouse monoclonal anti-beta-Actin Santa Cruz Biotechnology Cat# sc-47778 AB_626632 Mouse monoclonal anti-alpha-tubulin Sigma-Aldrich Cat# 00020911 AB_10013740 Peroxidase AffiniPure Goat Anti-Mouse IgG, Light Chain Specific for Western blotting after IP Jackson ImmunoResearch Cat# 115-035-174 AB_2338512 Peroxidase IgG Fraction Monoclonal Mouse Anti-Rabbit IgG, Light Chain Specific Jackson ImmunoResearch 211-032-171 AB_2339149 Mouse IgG Isotype Control Thermo Fisher Cat# 31903 AB_10959891 Rabbit IgG Isotype Control Thermo Fisher Cat# 31235 AB_243593 Bacterial and Virus Strains DH5a competent cells NEW ENGLAND BioLabs Cat# C2987H BL21(DE3) competent cells NEW ENGLAND BioLabs Cat# C2527H Stbl3 competent cells Thermo Fisher Cat# A10469 Biological Samples Hepatocellular carcinoma (HCC) tissue samples The First Affiliated Hospital of Nanchang University, Jiangxi, China.

Techniques: Western Blot, Recombinant, CCK-8 Assay, Plasmid Preparation, Software